Secondary Molecular Datasets

The CALERIE Genomic Data Resource includes genotype, DNA methylation, and RNAseq data for the CALERIE Trial. Illumina Global Array SNP genotype data for all CALERIE participants (from baseline blood DNA). Illumina EPICv1 DNA methylation data are available for up to three timepoints (baseline, 12mo, and 24mo) for blood, muscle, and adipose tissue samples. Already computed epigenetic clocks and estimated cell composition are available for the blood dataset. RNAseq data are available for muscle and adipose tissue samples. The data are described in detail in Ryan et al. 2025 Nat Aging (1), with related code and documentation available on GitHub.

Additional molecular data include the following (references):

PLASMA/BLOOD

  • Small molecule (micro) RNA sequencing (2)
  • Metabolomics (2)
  • Senescence associated secretory peptides (SASPs) (3)
  • NMR Lipidomics (4)
  • Telomeres (5)
  • IgG Glycome Pilot (6)
  • Plasminogen and plasminogen activator inhibitor-1 (PAI-1) concentrations; plasmin activity (7)

MUSCLE

  • Small molecule (micro) RNA sequencing (2)

ADIPOSE

  • Small molecule (micro) RNA sequencing (2)
  • Carbonylation (8): ProteomeXchange: DOI: 10.6019/PXD047731

All CALERIE data are intended to be housed with the NIA Aging Research Biobank. Access to data through the Aging Research Biobank is pending. In the meantime, investigators can access data through the Columbia University Geroscience Computational Core (cac_geroscience@cuimc.columbia.edu; https://www.belskylab.com/gcc). Data are available for research on human health and aging. Data access requests should include the following:

  • A summary of the proposed research (a paragraph or two is sufficient) – must confirm that purpose is non-commercial health research
  • A list of the datasets requested
  • A list of the investigators who will have access to the data (name, affiliation, position, email address)
  • Proof of IRB review of the study
  • Description of data security plans (what computing environment will the data be stored/analyzed in? is it encrypted, password protected, etc.)
  • Declaration that data will not be shared
  • Acknowledgement that you will reference NIH grant R01AG061378 in any publications or reports.
  • Please include a signature from the project PI in this document.

References:

  1. Ryan CP, Corcoran DL, Banskota N, Eckstein Indik C, Floratos A, Friedman R, Kobor MS, Kraus VB, Kraus WE, MacIsaac JL, Orenduff MC, Pieper CF, White JP, Ferrucci L, Horvath S, Huffman KM, Belsky DW. The CALERIE Genomic Data Resource. Nat Aging. 2025 Feb;5(2):320-331. doi: 10.1038/s43587-024-00775-0. Epub 2024 Dec 13. PMID: 39672986; PMCID: PMC11956694.
  2. Manuscript in preparation/review. Data access may be limited.
  3. Aversa Z, White TA, Heeren AA, Hulshizer CA, Saul D, Zhang X, Molina AJA, Redman LM, Martin CK, Racette SB, Huffman KM, Bhapkar M, Khosla S, Das SK, Fielding RA, Atkinson EJ, LeBrasseur NK. Calorie restriction reduces biomarkers of cellular senescence in humans. Aging Cell. 2024 Feb;23(2):e14038. doi: 10.1111/acel.14038. Epub 2023 Nov 14. PMID: 37961856; PMCID: PMC10861196.
  4. Huffman KM, Parker DC, Bhapkar M, Racette SB, Martin CK, Redman LM, Das SK, Connelly MA, Pieper CF, Orenduff M, Ross LM, Ramaker ME, Dorling JL, Rosen CJ, Shalaurova I, Otvos JD, Kraus VB, Kraus WE; CALERIE™ Investigators. Calorie restriction improves lipid-related emerging cardiometabolic risk factors in healthy adults without obesity: Distinct influences of BMI and sex from CALERIE™ a multicentre, phase 2, randomised controlled trial. EClinicalMedicine. 2022 Jan 3;43:101261. doi: 10.1016/j.eclinm.2021.101261. PMID: 35028547; PMCID: PMC8741476.
  5. Hastings WJ, Ye Q, Wolf SE, Ryan CP, Das SK, Huffman KM, Kobor MS, Kraus WE, MacIsaac JL, Martin CK, Racette SB, Redman LM, Belsky DW, Shalev I. Effect of long-term caloric restriction on telomere length in healthy adults: CALERIE™ 2 trial analysis. Aging Cell. 2024 Jun;23(6):e14149. doi: 10.1111/acel.14149. Epub 2024 Mar 19. PMID: 38504468; PMCID: PMC11296136.
  6. Pribić T, Das JK, Đerek L, Belsky DW, Orenduff M, Huffman KM, Kraus WE, Deriš H, Šimunović J, Štambuk T, Hodžić AF, Kraus VB, Das SK, Racette SB, Banskota N, Ferruci L, Pieper C, Lewis NE, Lauc G, Krishnan S. A 2-year calorie restriction intervention reduces glycomic biological age biomarkers. medRxiv [Preprint]. 2024 Dec 5:2024.12.04.24318451. doi: 10.1101/2024.12.04.24318451. Update in: NPJ Aging. 2025 Aug 1;11(1):71. doi: 10.1038/s41514-025-00254-9. PMID: 39677441; PMCID: PMC11643172.
  7. Bareja A, Lee DE, Ho T, Waitt G, McKay LH, Hannou SA, Orenduff MC, McGreevy KM, Binder A, Ryan CP, Soderblom EJ, Belsky DW, Ferrucci L, Das JK, Banskota N, Kraus VB, Huebner JL, Kraus WE, Huffman KM, Baht GS, Horvath S, Parmer RJ, Miles LA, White JP. Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-RKT. Cell Rep. 2024 Mar 26;43(3):113881. doi: 10.1016/j.celrep.2024.113881. Epub 2024 Mar 4. PMID: 38442019; PMCID: PMC11075744.
  8. Merali C, Quinn C, Huffman KM, Pieper CF, Bogan JS, Barrero CA, Merali S. Sustained caloric restriction potentiates insulin action by activating prostacyclin synthase. Obesity (Silver Spring). 2024 Dec;32(12):2286-2298. doi: 10.1002/oby.24150. Epub 2024 Oct 17. PMID: 39420421; PMCID: PMC12034231.